Swine fever virus RT-PCR Detection Kit
Swine fever virus RT-PCR Detection Kit (Lyophilized)
This kit uses real-time fluorescent RT-PCR method to detect the RNA of Swine fever virus(CSFV) in tissue disease materials such as tonsils, lymph nodes and spleen and liquid disease materials such as vaccine and blood of pigs. It is suitable for the detection, diagnosis and epidemiological investigation of Swine fever virus. The kit is an ALL-READY PCR SYSTEM(Lyophilized), which contains the DNA amplification enzyme,reverse transcriptase, reaction buffer, specific primers and probes required for fluorescent PCR detection.
|CSFV PCR Mix||1 ×bottle (Lyophilized powder)||50Test||dNTPs, MgCl2, Primers, Probes,Reverse Transcriptase,Taq DNA polymerase|
|6×0.2ml 8 well-strip tube(Lyophilized)||48Test|
|Positive Control||1*0.2ml tube (lyophilized)||10Tests||
Plasmid containing CSFV specific fragments
|Dissolving solution||1.5 ml Cryotube||500uL||/|
|Negative Control||1.5 ml Cryotube||200uL||0.9%NaCl|
Storage & Shelf Life
(1)The kit can be transported at room temperature.
(2)The shelf life is 18 months at -20℃ and 12 months at 2℃~30℃.
(3)See the label on kit for the production date and expiration date.
(4)The lyophilized powder version reagent should be stored at -20℃ after dissolution and the repeated freeze -thaw should be less than 4 times.
GENECHECKER UF-150, UF-300 real-time fluorescence PCR instrument.
a) Bottle version:
b) 8 well-strip tube version:
|Step||Cycle||Temperature (℃)||Time||Fluorescence channel|
|60||10s||Collect FAM fluorescence|
*Note: The signals of FAM fluorescence channels will be collected at 60℃.
Interpreting Test Results
Interpretation of results
|Suspicious result, retest*|
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.