Africa swine fever virus PCR detection kit

Short Description:

This kit uses real-time fluorescent PCR method to detect the DNA of Africa swine fever virus(ASFV) in tissue disease materials such as tonsils, lymph nodes and spleen and liquid disease materials such as vaccine and blood of pigs.


Product Detail

Product Tags

Product Name

Africa swine fever virus PCR detection kit (Lyophilized)

Size

48tests/kit, 50tests/kit

Intended Use

This kit uses real-time fluorescent PCR method to detect the DNA of Africa swine fever virus(ASFV) in tissue disease materials such as tonsils, lymph nodes and spleen and liquid disease materials such as vaccine and blood of pigs. It is suitable for the detection, diagnosis and epidemiological investigation of Africa swine fever virus. The kit is an ALL-READY PCR SYSTEM(Lyophilized), which contains the DNA amplification enzyme, reaction buffer, specific primers and probes required for fluorescent PCR detection..

Product Contents

Components Package specification Ingredient
ASFV PCR Mix 1 ×bottle                  (Lyophilized powder)  50Test dNTPs, MgCl2, Primers, Probes,Reverse Transcriptase,Taq DNA polymerase
6×0.2ml 8 well-strip tube(Lyophilized) 48Test
Positive Control 1*0.2ml tube (lyophilized)  10Tests

Plasmid containing ASFV specific fragments

Dissolving solution 1.5 ml Cryotube 500uL /
Negative Control 1.5 ml Cryotube 200uL 0.9%NaCl

Storage & Shelf Life

(1)The kit can be transported at room temperature.

(2)The shelf life is 18 months at -20℃ and 12 months at 2℃~30℃.

(3)See the label on kit for the production date and expiration date.

(4)The lyophilized powder version reagent should be stored at -20℃ after dissolution and the repeated freeze -thaw should be less than 4 times.

Instruments

GENECHECKER UF-150, UF-300 real-time fluorescence PCR instrument.

Operation Diagram

a) Bottle version:

1

b)  8 well-strip tube version:

2

Pcr Amplification

Recommended Setting

Step     Cycle Temperature (℃)   Time Fluorescence channel
1 1 95 2min  
2 40 95 5s  
60 10s Collect FAM fluorescence

*Note:  The signals of FAM fluorescence channels will be collected at 60℃.

Interpreting Test Results

Channel

Interpretation of results

FAM Channel

Ct≤35

ASFV Positive

Undet

ASFV Negative

35<Ct≤40

Suspicious result, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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