E.coli O157:H7 PCR detection kit
E.coli O157:H7 PCR detection kit (Lyophilized)
Escherichia coli O157:H7 (E.coli O157:H7) is a gram-negative bacterium belonging to the genus Enterobacteriaceae, which produces a large amount of Vero toxin. Clinically, it usually occurs suddenly with severe abdominal pain and watery diarrhea, followed by hemorrhagic diarrhea a few days later, which can lead to fever or no fever, and death in severe cases. This kit is suitable for the qualitative detection of Escherichia coli O157:H7 in food, water samples, feces, vomit, bacterium-enhancing liquid and other samples using the principle of real-time PCR.The kit is an ALL-READY PCR SYSTEM(Lyophilized), which contains the DNA amplification enzyme, reaction buffer, specific primers and probes required for fluorescent PCR detection..
|E.coli O157:H7 PCR Mix||1 ×bottle (Lyophilized powder)||50Test||dNTPs, MgCl2, Primers, Probes,Reverse Transcriptase,Taq DNA polymerase|
|6×0.2ml 8 well-strip tube(Lyophilized)||48Test|
|Positive Control||1*0.2ml tube (lyophilized)||10Tests||
Plasmid containing E.coli O157:H7 specific fragments
|Dissolving solution||1.5 ml Cryotube||500uL||/|
|Negative Control||1.5 ml Cryotube||200uL||0.9%NaCl|
Storage & Shelf Life
(1)The kit can be transported at room temperature.
(2)The shelf life is 18 months at -20℃ and 12 months at 2℃~30℃.
(3)See the label on kit for the production date and expiration date.
(4)The lyophilized powder version reagent should be stored at -20℃ after dissolution and the repeated freeze -thaw should be less than 4 times.
GENECHECKER UF-150, UF-300 real-time fluorescence PCR instrument.
a) Bottle version:
b) 8 well-strip tube version:
|Step||Cycle||Temperature (℃)||Time||Fluorescence channel|
|60||10s||Collect FAM fluorescence|
*Note: The signal of FAM fluorescence channel will be collected at 60℃.
Interpreting Test Results
Interpretation of results
|E.coli O157:H7 Positive|
|E.coli O157:H7 Negative|
|Suspicious resut, retest*|
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.