中文E.coli O157:H7 PCR detection kit
Product Name
E.coli O157:H7 PCR detection kit (Lyophilized)
Size
48tests/kit, 50tests/kit
Intended Use
Escherichia coli O157:H7 (E.coli O157:H7) is a gram-negative bacterium belonging to the genus Enterobacteriaceae, which produces a large amount of Vero toxin. Clinically, it usually occurs suddenly with severe abdominal pain and watery diarrhea, followed by hemorrhagic diarrhea a few days later, which can lead to fever or no fever, and death in severe cases. This kit is suitable for the qualitative detection of Escherichia coli O157:H7 in food, water samples, feces, vomit, bacterium-enhancing liquid and other samples using the principle of real-time PCR.The kit is an ALL-READY PCR SYSTEM(Lyophilized), which contains the DNA amplification enzyme, reaction buffer, specific primers and probes required for fluorescent PCR detection..
Product Contents
| Components | Package | specification | Ingredient |
| E.coli O157:H7 PCR Mix | 1 ×bottle (Lyophilized powder) | 50Test | dNTPs, MgCl2, Primers, Probes,Reverse Transcriptase,Taq DNA polymerase |
| 6×0.2ml 8 well-strip tube(Lyophilized) | 48Test | ||
| Positive Control | 1*0.2ml tube (lyophilized) | 10Tests |
Plasmid containing E.coli O157:H7 specific fragments |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Storage & Shelf Life
(1)The kit can be transported at room temperature.
(2)The shelf life is 18 months at -20℃ and 12 months at 2℃~30℃.
(3)See the label on kit for the production date and expiration date.
(4)The lyophilized powder version reagent should be stored at -20℃ after dissolution and the repeated freeze -thaw should be less than 4 times.
Instruments
GENECHECKER UF-150, UF-300 real-time fluorescence PCR instrument.
Operation Diagram
a) Bottle version:
b) 8 well-strip tube version:
Pcr Amplification
Recommended Setting
| Step | Cycle | Temperature (℃) | Time | Fluorescence channel |
| 1 | 1 | 95 | 2min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Collect FAM fluorescence |
*Note: The signal of FAM fluorescence channel will be collected at 60℃.
Interpreting Test Results
|
Channel |
Interpretation of results |
|
FAM Channel |
|
|
Ct≤35 |
E.coli O157:H7 Positive |
|
Undet |
E.coli O157:H7 Negative |
|
35<Ct≤40 |
Suspicious resut, retest* |
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.
