Salmonella PCR Detection Kit

Short Description:

Salmonella belongs to the Enterobacteriaceae and Gram-negative enterobacteria. Salmonella is a common food-borne pathogen and ranks first in bacterial food poisoning.


Product Detail

Product Tags

Product Name

Salmonella PCR Detection Kit (lyophilized)

Size

48tests/kit, 50tests/kit

Intended Use

Salmonella belongs to the Enterobacteriaceae and Gram-negative enterobacteria. Salmonella is a common food-borne pathogen and ranks first in bacterial food poisoning. The main symptoms of food poisoning caused by Salmonella are nausea, vomiting, abdominal pain, headache, chills and diarrhea. This kit uses the principle of real-time fluorescent PCR and is suitable for the qualitative detection of Salmonella in food, water samples, feces, vomit, and enrichment liquid.

Product Contents

Components Package specification Ingredient
PCR Mix 1 ×bottle                  (Lyophilized powder)  50Test dNTPs, MgCl2, Primers, Probes,Taq DNA polymerase
6×0.2ml 8 well-strip tube(Lyophilized) 48Test
Positive Control 1*0.2ml tube (lyophilized)  10Tests

Plasmid or Pseudovirus containing specific fragments

Dissolving solution 1.5 ml Cryotube 500uL /
Negative Control 1.5 ml Cryotube 100uL 0.9%NaCl

Storage & Shelf Life

(1)The kit can be transported at room temperature.

(2)The shelf life is 18 months at -20℃ and 12 months at 2℃~30℃.

(3)See the label on kit for the production date and expiration date.

(4)The lyophilized powder version reagent should be stored at -20℃ after dissolution and the repeated freeze -thaw should be less than 4 times.

Instruments

GENECHECKER UF-150, UF-300 real-time fluorescence PCR instrument.

Operation Diagram

a) Bottle version:

1

b)  8 well-strip tube version:

2

Pcr Amplification

Recommended Setting

Step

Cycle

Temperature (℃)

Time

Fluorescence channel

1

1

95

2min

/

2

40

95

5s

/

60

10s

Collect FAM fluorescence

Interpreting Test Results

Channel

Interpretation of results

FAM Channel

Ct≤35

Salmonella Positive

Undet

Salmonella Negative

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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